Difference between revisions of "Part:BBa K3753013"

Revision as of 14:09, 16 October 2021

UAS_TEF-pTEF

UAS_TEF-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from translational elongation factor EF-1 α tef1, and the core promoter tef2. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 629
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 629
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 629
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 629
Illegal NgoMIV site found at 1151
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 167
Illegal BsaI.rc site found at 378
Illegal BsaI.rc site found at 587

Revision as of 14:07, 16 October 2021 (view source) Registry (Talk \| contribs)		Revision as of 14:09, 16 October 2021 (view source) RI2020 (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K3753013 short</partinfo>		<partinfo>BBa_K3753013 short</partinfo>

−	UAS<sub>TEF</sub>-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from ~~Translational~~ elongation factor EF-1 α <em>tef1</em>, and the core promoter <em>tef2</em>. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.	+	UAS<sub>TEF</sub>-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from translational elongation factor EF-1 α <em>tef1</em>, and the core promoter <em>tef2</em>. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.