Difference between revisions of "Part:BBa K3893027"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3893027 short</partinfo>
 
<partinfo>BBa_K3893027 short</partinfo>
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[[File:T--Ecuador--K3893027_sbolv.png|200px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the part ]]
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AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.
 
AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.
  
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This composite part, together with [https://parts.igem.org/Part:BBa_K3893027 K3893027], is a component of the intermediate part [https://parts.igem.org/Part:BBa_K3893028]  to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030]) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.
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This composite part, together with [https://parts.igem.org/Part:BBa_K3893026 K3893026], is a component of the intermediate part [https://parts.igem.org/Part:BBa_K3893028]  to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030]) of our Agrobactory 593. It is used to express the lysis protein Phi174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.
  
 
This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:  
 
This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:  
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<ul>
 
<ul>
  
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<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] constitutive promoter in its Golden Braid compatible version <html></li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656003 BBa_K2656003]: the [https://parts.igem.org/Part:BBa_R0062 R0062] inducible pLux promoter in its Golden Braid compatible version <html></li>
  
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<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] strong ribosome biding site in its Golden Braid compatible version <html></li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656010 BBa_K2656010]: the [https://parts.igem.org/Part:BBa_B0032 B0032] weak ribosome biding site in its Golden Braid compatible version <html></li>
  
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<li></html> [https://parts.igem.org/Part:BBa_K2656019 BBa_K2656019]: the [https://parts.igem.org/Part:BBa_K2656019 K2656013]  luxI sequence in its Golden Braid standardized version <html></li>
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<li></html> [https://parts.igem.org/Part:BBa_K2656015 BBa_K2656015]: the Pho174 lysis protein <html></li>
  
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
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</html>
 
</html>
  
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Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161 C0161]adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon-optimized for E. coli.
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Part BBa_K2656015 is the lysis gene from the enterobacteria phage phiX174 coding sequence. The lysis gene E of coliphage PhiX174 encodes a 91-amino acid (aa) residue protein of approximately 10 kDa that mediates inhibition of peptidoglycan biosynthesis of Gram-negative bacteria membrane.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:09, 16 October 2021


AHL induced expression of Lysis protein.

domestication.
Figure 1. SBOL Visual representation of the part


AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893026, is a component of the intermediate part [1] to assemble the release system device ([2]) of our Agrobactory 593. It is used to express the lysis protein Phi174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

  • BBa_K2656003: the R0062 inducible pLux promoter in its Golden Braid compatible version
  • BBa_K2656010: the B0032 weak ribosome biding site in its Golden Braid compatible version
  • BBa_K2656015: the Pho174 lysis protein
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656015 is the lysis gene from the enterobacteria phage phiX174 coding sequence. The lysis gene E of coliphage PhiX174 encodes a 91-amino acid (aa) residue protein of approximately 10 kDa that mediates inhibition of peptidoglycan biosynthesis of Gram-negative bacteria membrane.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]