Difference between revisions of "Part:BBa K3735001:Design"
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===Source=== | ===Source=== | ||
| − | + | SefA is a protein from Thaurea selenatis (a β-proteobacteria).During selenate respiration by Thauera selenatis, the reduction of selenate results in the formation of intracellular selenium (Se) deposits that are ultimately secreted as Se nanospheres. Studies have found that SefA is closely related to the deposition of elemental selenium. Then the gene was cloned and expressed in Escherichia coli. The gene sequence of this part was obtained from NCBI and its GenBank Accession number is: HQ380173.1. | |
===References=== | ===References=== | ||
Revision as of 12:51, 16 October 2021
Coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1476
Illegal BglII site found at 2793 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 268
Illegal NgoMIV site found at 583
Illegal NgoMIV site found at 709
Illegal NgoMIV site found at 1090
Illegal NgoMIV site found at 1513
Illegal NgoMIV site found at 2614
Illegal NgoMIV site found at 2863
Illegal AgeI site found at 610
Illegal AgeI site found at 1156
Illegal AgeI site found at 1312
Illegal AgeI site found at 1453
Illegal AgeI site found at 1876
Illegal AgeI site found at 2233
Illegal AgeI site found at 2404 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
aa
Source
SefA is a protein from Thaurea selenatis (a β-proteobacteria).During selenate respiration by Thauera selenatis, the reduction of selenate results in the formation of intracellular selenium (Se) deposits that are ultimately secreted as Se nanospheres. Studies have found that SefA is closely related to the deposition of elemental selenium. Then the gene was cloned and expressed in Escherichia coli. The gene sequence of this part was obtained from NCBI and its GenBank Accession number is: HQ380173.1.