Difference between revisions of "Part:BBa K3776001"
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<partinfo>BBa_K3776022 short</partinfo>
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<partinfo>BBa_K3776001 short</partinfo>
  
 
Designed by Vo et al. (2020), this protein complex includes transposases TnsA, TnsB, TnsC and Cas enzymes Cas 8, Cas7, Cas6. These proteins are guided by a crRNA prefix flanked by two repeats. Users should clone their crRNA between these two repeats. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks.  
 
Designed by Vo et al. (2020), this protein complex includes transposases TnsA, TnsB, TnsC and Cas enzymes Cas 8, Cas7, Cas6. These proteins are guided by a crRNA prefix flanked by two repeats. Users should clone their crRNA between these two repeats. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks.  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3776022 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3776001 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3776022 parameters</partinfo>
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<partinfo>BBa_K3776001 parameters</partinfo>
 
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Revision as of 10:17, 16 October 2021

DavB

Designed by Vo et al. (2020), this protein complex includes transposases TnsA, TnsB, TnsC and Cas enzymes Cas 8, Cas7, Cas6. These proteins are guided by a crRNA prefix flanked by two repeats. Users should clone their crRNA between these two repeats. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1488
    Illegal AgeI site found at 895
    Illegal AgeI site found at 1144
  • 1000
    COMPATIBLE WITH RFC[1000]