Difference between revisions of "Part:BBa K3776001"

 
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<partinfo>BBa_K3776022 short</partinfo>
  
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Designed by Vo et al. (2020), this protein complex includes transposases TnsA, TnsB, TnsC and Cas enzymes Cas 8, Cas7, Cas6. These proteins are guided by a crRNA prefix flanked by two repeats. Users should clone their crRNA between these two repeats. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3776022 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3776022 parameters</partinfo>
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Revision as of 10:17, 16 October 2021

Vrepeat

Designed by Vo et al. (2020), this protein complex includes transposases TnsA, TnsB, TnsC and Cas enzymes Cas 8, Cas7, Cas6. These proteins are guided by a crRNA prefix flanked by two repeats. Users should clone their crRNA between these two repeats. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]