Difference between revisions of "Part:BBa K3989002"
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This variant is generated via a directed evaluation system called <b>YESS 2.0</b> (Yeast Endoplasmic Reticulum (ER) Sequestration Screening). The structure is shown below.(Fig.A) Particularly, the sub-structure of at the binding site is shown as well.(Fig. B) The blue amino acid residues represent the mutated sites and the purple residue represent the binding and cutting site of the ligand. | This variant is generated via a directed evaluation system called <b>YESS 2.0</b> (Yeast Endoplasmic Reticulum (ER) Sequestration Screening). The structure is shown below.(Fig.A) Particularly, the sub-structure of at the binding site is shown as well.(Fig. B) The blue amino acid residues represent the mutated sites and the purple residue represent the binding and cutting site of the ligand. | ||
<figure> | <figure> | ||
− | <img src="https:// | + | <img src="https://parts.igem.org/wiki/images/b/bb/21_UZurich_eTEV_structure.jpeg"> |
<figcaption><b>Fig. A </b> Structural model of eTEV </figcaption> | <figcaption><b>Fig. A </b> Structural model of eTEV </figcaption> | ||
</figure> | </figure> |
Revision as of 10:17, 16 October 2021
eTEV, efficiency-enhanced TEV protease variant
eTEV protease is mutant from the original TEV protease. Seven amino acids(S3I, P8Q, S31T, E79G, T173A, V219R, A231V) are mutated in this variant and the catalytic efficiency is 2-fold higher than the original one.
Structure and binding site
This variant is generated via a directed evaluation system called YESS 2.0 (Yeast Endoplasmic Reticulum (ER) Sequestration Screening). The structure is shown below.(Fig.A) Particularly, the sub-structure of at the binding site is shown as well.(Fig. B) The blue amino acid residues represent the mutated sites and the purple residue represent the binding and cutting site of the ligand.
Performance
Besides this variant, the author also reported some other variants(for instance, BBa_K3989001) with different mutations. Different mutations in the TEV protease ligand-binding site show different catalytic efficincy. The results are shown in Fig. C.
Reference
1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.