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   <figcaption><b>Fig. 1 </b>. - Sub-structure at the binding site of eTEV. </figcaption>
 
   <figcaption><b>Fig. 1 </b>. - Sub-structure at the binding site of eTEV. </figcaption>
 
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===Reference===
 
===Reference===

Revision as of 09:41, 16 October 2021


eTEV, efficiency-enhanced TEV protease variant

eTEV protease is mutant from the original TEV protease. Seven amino acids(S3I, P8Q, S31T, E79G, T173A, V219R, A231V) are mutated in this variant and the catalytic efficiency is 2-fold higher than the original one.


Structure and binding site

Fig. 1 . - Sub-structure at the binding site of eTEV.

Reference

1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.