Difference between revisions of "Part:BBa K3866038"
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===Verification of Cloning=== | ===Verification of Cloning=== | ||
− | [[File:T--Thessaly--gfpgel.png|800px|thumb|none|<i><b>Figure 2.</b>: (C=Cut, U=Uncut) Restriction digestion of | + | [[File:T--Thessaly--gfpgel.png|800px|thumb|none|<i><b>Figure 2.</b>: (C=Cut, U=Uncut) Restriction digestion of α-sfGFP TU with: EcoRI, Expected bands: 2847bp, 801bp, 146bp, Positive result: C3, C4, C5</i>]] |
===Experimental Use and Experience=== | ===Experimental Use and Experience=== |
Revision as of 09:29, 16 October 2021
J23115:sfGFP:Terminator
Usage and Biology
This TU includes the sfGFP gene placed under the control of the constitutive Anderson promoter J23115 BBa_J23115.
Design Notes
The coding sequence was domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo.
Verification of Cloning
Experimental Use and Experience
This part showed functionality at the following parts: BBa_K3866029, BBa_K3866031
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 192
- 1000COMPATIBLE WITH RFC[1000]