Difference between revisions of "Part:BBa K3962348"

 
 
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<partinfo>BBa_K3962348 short</partinfo>
 
<partinfo>BBa_K3962348 short</partinfo>
  
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This construct is composed of constitutive promoter J23113 (from Anderson promoter collection) and toxin RelE. RelE/RelB is a type II toxin/antitoxin system originated from R1 plasmid in E. coli. In our project, we used this construct to create a balanced RelE/RelB toxin-antitoxin system for preventing horizontal gene transfer.  
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This construct is composed of constitutive promoter J23113 (from the Anderson promoter collection) and toxin RelE. RelE/RelB is a type II toxin-antitoxin system originating from the E. coli chromosome. In our project, we used this construct to create a balanced RelE/RelB toxin-antitoxin system for preventing horizontal gene transfer.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:03, 16 October 2021


J23113 promoter regulated constant expression of toxin RelE

This construct is composed of constitutive promoter J23113 (from the Anderson promoter collection) and toxin RelE. RelE/RelB is a type II toxin-antitoxin system originating from the E. coli chromosome. In our project, we used this construct to create a balanced RelE/RelB toxin-antitoxin system for preventing horizontal gene transfer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]