Difference between revisions of "Part:BBa K3962347"

Latest revision as of 08:02, 16 October 2021

J23113 promoter regulated constant expression of toxin CcdB

This construct is composed of constitutive promoter J23113 (from the Anderson promoter collection) and toxin CcdB. CcdB/CcdA is a type II toxin-antitoxin system originating from the E. coli chromosome. In our project, we used this construct to create a balanced CcdA/CcdB toxin-antitoxin system for preventing horizontal gene transfer.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 727
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 177

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 <partinfo>BBa_K3962347 short</partinfo>
-This construct is composed of constitutive promoter J23113 (from Anderson promoter collection) and toxin CcdB. CcdB/CcdA is a type II toxin/antitoxin system originated from E. coli. In our project, we used this construct to create a balanced HOK/SOK toxin-antitoxin system for preventing horizontal gene transfer.
+This construct is composed of constitutive promoter J23113 (from the Anderson promoter collection) and toxin CcdB. CcdB/CcdA is a type II toxin-antitoxin system originating from the E. coli chromosome. In our project, we used this construct to create a balanced CcdA/CcdB toxin-antitoxin system for preventing horizontal gene transfer.
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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3962347 SequenceAndFeatures</partinfo>