Difference between revisions of "Part:BBa K4047035"

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This coding region was sourced from the Synechococcus elongatus PCC 7942 Genbank genome sequence for class 1 fructose-1,6-bisphosphatase (FBPase) to maximize efficiency within the same organism as a chassis. This enzyme is natively expressed within the organism and plays a role in its central metabolism, catalyzing the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate.  
 
This coding region was sourced from the Synechococcus elongatus PCC 7942 Genbank genome sequence for class 1 fructose-1,6-bisphosphatase (FBPase) to maximize efficiency within the same organism as a chassis. This enzyme is natively expressed within the organism and plays a role in its central metabolism, catalyzing the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate.  
  
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This coding region has significant alignment with other categorized Synechococcus strains, as shown in the nucleotide blast result of the provided sequence through https://blast.ncbi.nlm.nih.gov/Blast.cgi Figure 1).  
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This coding region has significant alignment with other categorized Synechococcus strains, as shown in the nucleotide blast result of the provided sequence through https://blast.ncbi.nlm.nih.gov/Blast.cgi (Figure 1).  
  
  

Revision as of 03:45, 16 October 2021


Fructose-1,6-bisphosphatase

The gene fructose-1,6-bisphosphatase class I

Usage and Biology

This coding region was sourced from the Synechococcus elongatus PCC 7942 Genbank genome sequence for class 1 fructose-1,6-bisphosphatase (FBPase) to maximize efficiency within the same organism as a chassis. This enzyme is natively expressed within the organism and plays a role in its central metabolism, catalyzing the conversion of fructose-1,6-bisphosphate to fructose-6-phosphate.

This coding region has significant alignment with other categorized Synechococcus strains, as shown in the nucleotide blast result of the provided sequence through https://blast.ncbi.nlm.nih.gov/Blast.cgi (Figure 1).


Although this sequence possesses several nucleotide sequences that make it BioBrick incompatible, it is a vital central metabolism gene that future teams may want to work with. Relevant silent mutations could resolve this incompatibility and be a great contribution for future teams.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 493
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 493
    Illegal NotI site found at 746
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 36
    Illegal BglII site found at 656
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 493
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 22