Difference between revisions of "Part:BBa K3796217:Design"
Yang Feiyue (Talk | contribs) (→Source) |
Yang Feiyue (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We directly use the promoter and terminators carried by the plasmid to speed up our experiments. Users can change the promoter and terminators according to the plasmid they are using, as long as the promoter is inducible and has low leaky expression. | |
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===Source=== | ===Source=== | ||
Latest revision as of 03:10, 16 October 2021
Ptac-rbs-ndoA-terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We directly use the promoter and terminators carried by the plasmid to speed up our experiments. Users can change the promoter and terminators according to the plasmid they are using, as long as the promoter is inducible and has low leaky expression.
Source
nodA is from Bacillus subtilis subsp. subtilis str. 168. The rbs sequence information is from BBa_K2963006 and the sequence itself is synthetic. The promoter and terminators are carried by the plasmid pXMJ19 already.