Difference between revisions of "Part:BBa K3853011"
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This part consists of defective endonuclease dCas9(<partinfo>BBa_K3853003</partinfo>), modified immunoglobulin-like domain SpyCatcher and Ser/Gly linker(S/G linker). S/G linker is applied to link dCas9 with SpyCatcher. dCas9 can specifically target and bind dsDNA under the mediation of single-guide RNA (sgRNA), meanwhile, SpyCatcher is usually used for protein ligation. dCas9-SpyCatcher has been reported as connection system to assemble protein-nucleic-acid complex. We use <partinfo>BBa_K3853055</partinfo> to construct the expression system and to express and to purify the protein. | This part consists of defective endonuclease dCas9(<partinfo>BBa_K3853003</partinfo>), modified immunoglobulin-like domain SpyCatcher and Ser/Gly linker(S/G linker). S/G linker is applied to link dCas9 with SpyCatcher. dCas9 can specifically target and bind dsDNA under the mediation of single-guide RNA (sgRNA), meanwhile, SpyCatcher is usually used for protein ligation. dCas9-SpyCatcher has been reported as connection system to assemble protein-nucleic-acid complex. We use <partinfo>BBa_K3853055</partinfo> to construct the expression system and to express and to purify the protein. | ||
− | + | ===Biology=== | |
− | ===Usage | + | |
+ | ===Usage=== | ||
+ | |||
+ | ===Characterization=== | ||
+ | |||
+ | ===References=== | ||
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Revision as of 19:11, 15 October 2021
dCas9-SpyCatcher
This part consists of defective endonuclease dCas9(BBa_K3853003), modified immunoglobulin-like domain SpyCatcher and Ser/Gly linker(S/G linker). S/G linker is applied to link dCas9 with SpyCatcher. dCas9 can specifically target and bind dsDNA under the mediation of single-guide RNA (sgRNA), meanwhile, SpyCatcher is usually used for protein ligation. dCas9-SpyCatcher has been reported as connection system to assemble protein-nucleic-acid complex. We use BBa_K3853055 to construct the expression system and to express and to purify the protein.
Biology
Usage
Characterization
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3960
Illegal AgeI site found at 3304 - 1000COMPATIBLE WITH RFC[1000]