Difference between revisions of "Part:BBa K3853011"

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<partinfo>BBa_K3853011 short</partinfo>
 
<partinfo>BBa_K3853011 short</partinfo>
  
This part consists of defective endonuclease dCas9(<partinfo>BBa_K3853003</partinfo>), modified immunoglobulin-like domain SpyCatcher and Ser/Gly linker(S/G linker). S/G linker is applied to link dCas9 with SpyCatcher. dCas9 can specifically target and bind dsDNA under the mediation of single-guide RNA (sgRNA), meanwhile, SpyCatcher is usually used for protein ligation. dCas9-SpyCatcher has been reported as connection system to assemble protein-nucleic-acid complex. We use <partinfo>BBa_3853055</partinfo> to construct the expression system and to express and to purify the protein.
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This part consists of defective endonuclease dCas9(<partinfo>BBa_K3853003</partinfo>), modified immunoglobulin-like domain SpyCatcher and Ser/Gly linker(S/G linker). S/G linker is applied to link dCas9 with SpyCatcher. dCas9 can specifically target and bind dsDNA under the mediation of single-guide RNA (sgRNA), meanwhile, SpyCatcher is usually used for protein ligation. dCas9-SpyCatcher has been reported as connection system to assemble protein-nucleic-acid complex. We use <partinfo>BBa_K3853055</partinfo> to construct the expression system and to express and to purify the protein.
  
 
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Revision as of 18:37, 15 October 2021


dCas9-SpyCatcher

This part consists of defective endonuclease dCas9(BBa_K3853003), modified immunoglobulin-like domain SpyCatcher and Ser/Gly linker(S/G linker). S/G linker is applied to link dCas9 with SpyCatcher. dCas9 can specifically target and bind dsDNA under the mediation of single-guide RNA (sgRNA), meanwhile, SpyCatcher is usually used for protein ligation. dCas9-SpyCatcher has been reported as connection system to assemble protein-nucleic-acid complex. We use BBa_K3853055 to construct the expression system and to express and to purify the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3960
    Illegal AgeI site found at 3304
  • 1000
    COMPATIBLE WITH RFC[1000]