Difference between revisions of "Part:BBa K3788020"
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| + | <h1> expression of the timer device fused to the RFP protein, under the control of the LexA-controlled promoter </h1> | ||
Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. In this contruction RFP permit to understand the mechanism, and Cal isn't able to have his lysis activity. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc. | Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. In this contruction RFP permit to understand the mechanism, and Cal isn't able to have his lysis activity. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc. | ||
Revision as of 17:42, 15 October 2021
Expression of the timer device fused to the RFP protein
expression of the timer device fused to the RFP protein, under the control of the LexA-controlled promoter
Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. In this contruction RFP permit to understand the mechanism, and Cal isn't able to have his lysis activity. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 808
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1128
Illegal BamHI site found at 801
Illegal XhoI site found at 677 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 877
Illegal AgeI site found at 2129
Illegal AgeI site found at 2241 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 631