Difference between revisions of "Part:BBa K3788001:Experience"
| Line 11: | Line 11: | ||
https://2021.igem.org/wiki/images/a/a3/T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png | https://2021.igem.org/wiki/images/a/a3/T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png | ||
<p><u>Figure</u>. <b>Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning.</b> <i> | <p><u>Figure</u>. <b>Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning.</b> <i> | ||
| − | a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 | + | a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side). </i></p> |
Revision as of 16:56, 15 October 2021
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3788001
PCR amplification of flagCry11Aa gene :
The DNA sequence of flagcry11Aa was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 1956bp and a 2000bp band is observed. Amplification of DNAs sequences with primers were validated.
Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side).
User Reviews
UNIQ5c34e4f3b78c63a9-partinfo-00000000-QINU UNIQ5c34e4f3b78c63a9-partinfo-00000001-QINU