Difference between revisions of "Part:BBa K3788002"

Revision as of 16:50, 15 October 2021

6His-P20

BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis. This chaperon have a 6x Histine tag in N-ter to allow purification of the protein P20and specific detection by Western blot. The gene p20 was optimised for E. coli K12.

Expected size :

6his_p20 gene is 570bp

6His_P20 protein is 20 kDa

P20 chaperon:

Was discovered in B.thuringiensis, a p20 gene in operon with cry11Aa and <i<cyt1Aa</i>. Thus, the P20 protein was shown to be necessary for the formation of inclusion bodies of Cry11Aa and Cyt1Aa. P20 would allow a greater production of these toxins and allow their stability (Cyt1Aa would be protected from the proteolytic action before its maturation). The co-production of P20 and Cyt1Aa in E.coli would limit its toxic effects in the producer cell.

Sequence and Features