Difference between revisions of "Part:BBa K3788000"

Revision as of 16:48, 15 October 2021

StrepII-Cyt1Aa

BBa_K3788000 sequence is coding for Cyt1Aa, a toxins from Bacillus thuringiensis. This toxin has a Flag tag in N-ter to allow purification of the protein Cry11Aa and specific detection by Western blot. The gene cyt1Aa was optimised for E. coli K12.

Expected size :

strepII_cyt1Aa gene is 777bp

Strep_Cyt1Aa protein is 28,5 kDa

Mode of action of Cyt toxins:

Cyt (Cytolitic) toxins are produced in pro-toxin form, they will undergo proteolytic cleavage in N-ter and C-ter allowing the release of the active form of Cyt toxins. The mature forms will interact with the membrane lipids of the midgut cells and will insert themselves into the membrane, forming β-barrels. Cyt toxin are different in them domains.

Cyt1Aa would facilitate the binding of Cry11Aa to the BBMV receptor, by interaction of the loop α8, β4 and loop 2 regions of Cry11Aa at the level of the loop β6 – αE and part of β7 of Cyt1Aa. This interaction allows easy insertion of Cry11Aa into membranes.

Sequence and Features