Difference between revisions of "Part:BBa K3788004"
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<partinfo>BBa_K3788004 short</partinfo> | <partinfo>BBa_K3788004 short</partinfo> | ||
| − | The Part BBa_K3788004 is a part composed with BBa_K3788003 ( | + | <p>The Part BBa_K3788004 is a part composed with BBa_K3788003 (6His_<i>P20</i>__Flag_<i>cry11Aa</i>) and BBa_K3788000 (Strep_<i>cyt1Aa</i>). Theses 3 proteins proteins are <i>Bacillus thuringiensis</i> proteins. P20 chaperon have a histidin tag in Nter, Cry11Aa toxin have a Flag tag in Nter and Cyt1Aa toxin have a Strep tag II in Nter to allow to purified proteins P20, Cyt1Aa and Cry11Aa; and allow the detection and specific identification by WesterBlot. </p> |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 15:42, 15 October 2021
6His-P20, Flag-Cry11Aa and Strep-Cyt1Aa coding sequence
The Part BBa_K3788004 is a part composed with BBa_K3788003 (6His_P20__Flag_cry11Aa) and BBa_K3788000 (Strep_cyt1Aa). Theses 3 proteins proteins are Bacillus thuringiensis proteins. P20 chaperon have a histidin tag in Nter, Cry11Aa toxin have a Flag tag in Nter and Cyt1Aa toxin have a Strep tag II in Nter to allow to purified proteins P20, Cyt1Aa and Cry11Aa; and allow the detection and specific identification by WesterBlot.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 154
Illegal XhoI site found at 2438 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]