Difference between revisions of "Part:BBa K3866038"
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The coding sequence was domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in a PDGB3_α1 vector and has overhangs compatible for GoldenBraid cloning. | The coding sequence was domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in a PDGB3_α1 vector and has overhangs compatible for GoldenBraid cloning. | ||
| − | [[Image:T--Thessaly-- | + | [[Image:T--Thessaly--J23115-sfGFP-term.png|800px|thumb|none|<I><b>Figure 1.</b> The first level α module of the Propionate Production: α1:ParaBAD:RBS-smb-Double terminator </i>]] |
===Verification of Cloning=== | ===Verification of Cloning=== | ||
Revision as of 14:43, 15 October 2021
J23115:sfGFP:Terminator
Usage and Biology
This TU includes the sbm gene placed under the control of the arabinosed-induced promoter BBa_K3866000. Initially, the level 1 cloning was performed using the trc promoter BBa_K3866001, though we weren't able to isolate a colony with the desired construct, as there is a possibility that the bacteria's metabolism went out of balance so no colonies survived to be chosen for screening. Instead we used the arabinose-indused promoter, which seemed to be working as expected.
Design Notes
The coding sequence was domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in a PDGB3_α1 vector and has overhangs compatible for GoldenBraid cloning.
Verification of Cloning
Experimental Use and Experience
This part showed functionality at the following parts: BBa_K3866029, BBa_K3866031