Difference between revisions of "Part:BBa K3788001:Design"
| Line 18: | Line 18: | ||
===References=== | ===References=== | ||
| − | |||
<p> - Bravo, A., Gill, S. S. & Soberón, M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49, 423–435 (2007).</p> | <p> - Bravo, A., Gill, S. S. & Soberón, M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49, 423–435 (2007).</p> | ||
<p> - Hua, G., Masson, L., Jurat-Fuentes, J. L., Schwab, G. & Adang, M. J. Binding Analysis of Bacillus thuringiensis Cry δ-Endotoxins Using Brush Border Membrane Vesicles of Ostrinia nubilalis. Appl. Environ. Microbiol. 67, 872–879 (2001). </p> | <p> - Hua, G., Masson, L., Jurat-Fuentes, J. L., Schwab, G. & Adang, M. J. Binding Analysis of Bacillus thuringiensis Cry δ-Endotoxins Using Brush Border Membrane Vesicles of Ostrinia nubilalis. Appl. Environ. Microbiol. 67, 872–879 (2001). </p> | ||
Latest revision as of 14:40, 15 October 2021
Flag-Cry11Aa
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1854
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.
This part is from the BBa_K2938005 part, the sequence was optimised for E. coli
Source
References
- Bravo, A., Gill, S. S. & Soberón, M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49, 423–435 (2007).
- Hua, G., Masson, L., Jurat-Fuentes, J. L., Schwab, G. & Adang, M. J. Binding Analysis of Bacillus thuringiensis Cry δ-Endotoxins Using Brush Border Membrane Vesicles of Ostrinia nubilalis. Appl. Environ. Microbiol. 67, 872–879 (2001).