Difference between revisions of "Part:BBa K3962340:Design"

 
 
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===Design Notes===
 
===Design Notes===
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As it was used as reference of fluorescent protein expression, the subparts including the promoter, RBS, reporter and terminator were the most frequently used parts in the registry.  
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We designed this construct to calibrate the expression of pBAD to a standardized constitutive promoter. Codon optimization was performed for the expression of the gene in E. coli.  
  
  

Latest revision as of 14:19, 15 October 2021


pBAD promoter with mCherry for inducible expression of reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We designed this construct to calibrate the expression of pBAD to a standardized constitutive promoter. Codon optimization was performed for the expression of the gene in E. coli.


Source

All subparts are from iGEM registry.

References