Difference between revisions of "Part:BBa K3788000:Design"

 
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===Design Notes===
 
===Design Notes===
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After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.
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<p>After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.</p>
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<p>This part is from the <b>BBa_K2938003 part</b>, the sequence was optimised for <i> E. coli </i></p>
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<p> <u> reference : </u> </p>
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<p> Bravo, A., Gill, S. S. & Soberón, M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49, 423–435 (2007).</p>
  
  

Revision as of 14:16, 15 October 2021


StrepII-Cyt1Aa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.

This part is from the BBa_K2938003 part, the sequence was optimised for E. coli

reference :

Bravo, A., Gill, S. S. & Soberón, M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49, 423–435 (2007).


Source

The cyt1Aa sequence was optimised from the part : Part:BBa_K2938003 The sequence codding for Strep tag II was added in 5' of the Part:BBa_K2938003 optimised

References