Difference between revisions of "Part:BBa K3930029"
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
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<p>The promoter Ptrc-riboswitch E comes from the publication of Nakahira et al. 2013. It comprises a double regulation of the downstream gene expression. The first is a transcriptional regulation by the IPTG-inducible TRC promoter. The second regulation is post-transcriptional, as a theophylline-inducible aptamer sequesters the RBS essential for mRNA translation. This promoter has been originally described for cyanobacterium  <i>Synechococcus elongatus</i> PCC 7942 control of gene expression.</p>
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<p>The promoter Ptrc-riboswitch E comes from the publication of Nakahira et al. 2013. It comprises a double regulation of the downstream gene expression. The first is a transcriptional regulation by the IPTG-inducible TRC promoter. The second regulation is post-transcriptional, as a theophylline-inducible aptamer sequesters the RBS essential for mRNA translation. This promoter has been originally described for control of gene expression in the cyanobacterium  <i>Synechococcus elongatus</i> PCC 7942 .</p>
 
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<h2>References</h2>
 
<h2>References</h2>

Revision as of 13:07, 15 October 2021


IPTG and theophylline inducible promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

The promoter Ptrc-riboswitch E comes from the publication of Nakahira et al. 2013. It comprises a double regulation of the downstream gene expression. The first is a transcriptional regulation by the IPTG-inducible TRC promoter. The second regulation is post-transcriptional, as a theophylline-inducible aptamer sequesters the RBS essential for mRNA translation. This promoter has been originally described for control of gene expression in the cyanobacterium Synechococcus elongatus PCC 7942 .


References

  1. Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. 2013. Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942. Plant and Cell Physiology. 54(10):1724–1735. doi:10.1093/pcp/pct115.
    2.