Difference between revisions of "Part:BBa K3930025"
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
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<p>The NsrR cassette is composed by the promoter TEF1, the resistance gene <i>nsrR</i> and the transcription terminator of CYC1. The resistance gene is based on the part (BBa_K3629011), but it was codon optimized for expression into <i>S.cerevisiae</i>.</p>
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<p>The NsrR cassette is composed of the promoter TEF1, the resistance gene <i>nsrR</i> and the transcription terminator of CYC1. The resistance gene is based on the part (BBa_K3629011), but it was codon optimized for expression into <i>S.cerevisiae</i>.</p>
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<p>This gene codes for a Nourseothricin acetyltransferase , which inactivate the nourseothricin by adding a acetyl on it. Therefore, this cassette aload the selection of Yeast transformant upon addition of nourseothricin into the media of culture. </p>  
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<p>This gene codes for a nourseothricin acetyltransferase , which inactivates the nourseothricin by adding a acetyl. Therefore, this cassette allows the selection of yeast transformants upon addition of nourseothricin into the media. </p>  
  
 
<h3>Part characterization</h3>
 
<h3>Part characterization</h3>
  
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<p> The part (BBa_K3930025) was used to select the right genomic integration of the part (BBa_K3930003). Transformants were plated onto YPD + 250 &mu;g.ml-1 nourseothricin Petri dish. The Toulouse INSA UPS team manage to select a transformant with this selection marker (for more details, check the (BBa_K3930003) part page) </p>
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<p> The part (BBa_K3930025) was used to select for genomic integration of part (BBa_K3930003). Transformants were plated onto YPD Petri plates containing 250 &mu;g.ml-1 nourseothricin. The Toulouse INSA UPS team managed to select transformants with this selection marker (for more details, check the (BBa_K3930003) part page) </p>
  
 
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Revision as of 12:51, 15 October 2021


Nourseothricin selective cassette Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 710
    Illegal AgeI site found at 428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168

Introduction

The NsrR cassette is composed of the promoter TEF1, the resistance gene nsrR and the transcription terminator of CYC1. The resistance gene is based on the part (BBa_K3629011), but it was codon optimized for expression into S.cerevisiae.

This gene codes for a nourseothricin acetyltransferase , which inactivates the nourseothricin by adding a acetyl. Therefore, this cassette allows the selection of yeast transformants upon addition of nourseothricin into the media.

Part characterization

The part (BBa_K3930025) was used to select for genomic integration of part (BBa_K3930003). Transformants were plated onto YPD Petri plates containing 250 μg.ml-1 nourseothricin. The Toulouse INSA UPS team managed to select transformants with this selection marker (for more details, check the (BBa_K3930003) part page)