Difference between revisions of "Part:BBa K3972004"
Ingrid Kolen (Talk | contribs) |
Ingrid Kolen (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<partinfo>BBa_K3972004 short</partinfo> | <partinfo>BBa_K3972004 short</partinfo> | ||
| − | This composite part is a combination of the parts [https://parts.igem.org/Part:BBa_K3972001 BBa_K3972001] and [https://parts.igem.org/Part:BBa_K3972002 BBa_K3972002], with additional functional parts [https://parts.igem.org/Part:BBa_R0040 BBa_R0040], [https://parts.igem.org/Part:BBa_K3332034 BBa_K3332034] and [https://parts.igem.org/Part:BBa_K2507019 BBa_K2507019]. The ARG1 proteins are controlled by the | + | This composite part is a combination of the parts [https://parts.igem.org/Part:BBa_K3972001 BBa_K3972001] and [https://parts.igem.org/Part:BBa_K3972002 BBa_K3972002], with additional functional parts [https://parts.igem.org/Part:BBa_R0040 BBa_R0040], [https://parts.igem.org/Part:BBa_K3332034 BBa_K3332034] and [https://parts.igem.org/Part:BBa_K2507019 BBa_K2507019]. The ARG1 proteins are controlled by the pTtrB185-269 promoter. This promoter is activated by TtrR in the presence of TtrS and tetrathionate. In the presence of tetrathionate, TtrS is activated and in its turn TtrS activates TtrR. TtrR binds to the pTtrB185-269 promoter and induces expression of the ARG1 gas vesicle proteins |
| + | [[File:T—TU_Eindhoven--designA1.png|600px|]] | ||
| + | |||
| + | ''Figure 1. The mechanism of the composite part. '' | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part is optimized for expression of ARG1 in E. coli cells. This part was successfully transformed after multiple attempts in XL10-Gold and Top10 cells. The cotransformation together with TtrS did also result in colonies on plates with BL21 (DE3), Top10 and XL10-Gold cells. Unfortunately, there was no time left for testing this part with ultrasound equipment. | ||
| + | |||
| + | |||
| + | ==Characterization== | ||
| + | <strong>Expression</strong> | ||
| + | <br> | ||
| + | This part is optimized for expression of ARG1 in E. coli cells. This part was successfully transformed after multiple attempts in XL10-Gold and Top10 cells. The cotransformation together with TtrS did also result in colonies on plates with BL21 (DE3), Top10 and XL10-Gold cells. Unfortunately, there was no time left for testing this part with ultrasound equipment. | ||
| + | |||
| + | |||
| + | [[File: T--TU-Eindhoven--combiB ARG.jpeg|200px|]] | ||
| + | ''Figure 2. Agar plate with transfected ARG1 with the insert in XL10-Gold cells.'' | ||
| + | |||
| + | |||
| + | [[File:T—TU_Eindhoven--combiB.png|200px|]] | ||
| + | ''Figure 3. Agar plate with no cotransfected ARG1, TtrR and TtrS in BL21 (DE3).'' | ||
| + | |||
| − | + | ==Sequence and Features== | |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3972004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3972004 SequenceAndFeatures</partinfo> | ||
Revision as of 11:49, 15 October 2021
Tetrathionate sensor with transferred TtrR and pTtrB185-269 activated ARG1
This composite part is a combination of the parts BBa_K3972001 and BBa_K3972002, with additional functional parts BBa_R0040, BBa_K3332034 and BBa_K2507019. The ARG1 proteins are controlled by the pTtrB185-269 promoter. This promoter is activated by TtrR in the presence of TtrS and tetrathionate. In the presence of tetrathionate, TtrS is activated and in its turn TtrS activates TtrR. TtrR binds to the pTtrB185-269 promoter and induces expression of the ARG1 gas vesicle proteins
Figure 1. The mechanism of the composite part.