Difference between revisions of "Part:BBa K3972004"

 
Line 3: Line 3:
 
<partinfo>BBa_K3972004 short</partinfo>
 
<partinfo>BBa_K3972004 short</partinfo>
  
−
This composite part is a combination of the parts BBa_K3972001 and BBa_K3972002. The ARG1 proteins are activated by the PttrB185-269 promotor. This promotor is activated by TtrR in the presence of TtrS and tetrathionate. Therefore, in the presence of tetrathionate, TtrS is activated and on its turn activates TtrR. TtrR binds to the PttrB185-269 promotor and induces expression of the ARG1 proteins.  
+
This composite part is a combination of the parts [https://parts.igem.org/Part:BBa_K3972001 BBa_K3972001] and [https://parts.igem.org/Part:BBa_K3972002 BBa_K3972002], with additional functional parts [https://parts.igem.org/Part:BBa_R0040 BBa_R0040], [https://parts.igem.org/Part:BBa_K3332034 BBa_K3332034] and [https://parts.igem.org/Part:BBa_K2507019 BBa_K2507019]. The ARG1 proteins are controlled by the PttrB185-269 promoter. This promoter is activated by TtrR in the presence of TtrS and tetrathionate. In the presence of tetrathionate, TtrS is activated and in its turn TtrS activates TtrR. TtrR binds to the pTtrB185-269 promoter and induces expression of the ARG1 gas vesicle proteins.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:39, 15 October 2021


Tetrathionate sensor with transferred TtrR and pTtrB185-269 activated ARG1

This composite part is a combination of the parts BBa_K3972001 and BBa_K3972002, with additional functional parts BBa_R0040, BBa_K3332034 and BBa_K2507019. The ARG1 proteins are controlled by the PttrB185-269 promoter. This promoter is activated by TtrR in the presence of TtrS and tetrathionate. In the presence of tetrathionate, TtrS is activated and in its turn TtrS activates TtrR. TtrR binds to the pTtrB185-269 promoter and induces expression of the ARG1 gas vesicle proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1658
    Illegal EcoRI site found at 4811
    Illegal XbaI site found at 1409
    Illegal PstI site found at 404
    Illegal PstI site found at 4668
    Illegal PstI site found at 4773
    Illegal PstI site found at 5151
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1658
    Illegal EcoRI site found at 4811
    Illegal NheI site found at 1543
    Illegal PstI site found at 404
    Illegal PstI site found at 4668
    Illegal PstI site found at 4773
    Illegal PstI site found at 5151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1658
    Illegal EcoRI site found at 4811
    Illegal BglII site found at 2162
    Illegal BamHI site found at 5567
    Illegal XhoI site found at 5255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1658
    Illegal EcoRI site found at 4811
    Illegal XbaI site found at 1409
    Illegal PstI site found at 404
    Illegal PstI site found at 4668
    Illegal PstI site found at 4773
    Illegal PstI site found at 5151
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1658
    Illegal EcoRI site found at 4811
    Illegal XbaI site found at 1409
    Illegal PstI site found at 404
    Illegal PstI site found at 4668
    Illegal PstI site found at 4773
    Illegal PstI site found at 5151
    Illegal NgoMIV site found at 2613
    Illegal NgoMIV site found at 3015
    Illegal AgeI site found at 334
    Illegal AgeI site found at 5534
  • 1000
    COMPATIBLE WITH RFC[1000]