Difference between revisions of "Part:BBa K3753003:Design"

 
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===References===
 
===References===
 +
 +
Dominik Mumberg and Rolf Müller and Martin Funk. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds[J]. Gene, 1995, 156(1) : 119-122.
 +
Schirmaier F and Philippsen P. Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae.[J]. The EMBO journal, 1984, 3(13) : 3311-5.

Revision as of 08:02, 15 October 2021


pTEF2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TEF2 maps on chromosome II in the yeast Saccharomyces cerevisiae. pTEF(TEF2) was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 1000bp sequence upstream of the TEF2 coding sequence, which is a putative TEF2 promoter.


Source

Saccharomyces cerevisiae

References

Dominik Mumberg and Rolf Müller and Martin Funk. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds[J]. Gene, 1995, 156(1) : 119-122. Schirmaier F and Philippsen P. Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae.[J]. The EMBO journal, 1984, 3(13) : 3311-5.