Difference between revisions of "Part:BBa K3895007"
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==Construction== | ==Construction== | ||
Keratinase KerAVDZ50 (<partinfo>K3895003</partinfo>) is an extracellular serine thiol alkaline protease from the ''Actinomadura viridilutea'' strain, which can be isolated from the Algerian fishing port [1]. As a thermo-alkaline keratinases, it can function at a pH range of 7–12 and a temperature range of 35–80 °C. 6x His-tags were added to both sides of the sequence for purification and constructed into PET-28a(+). The constitutive T7 promoter (<partinfo>I719005</partinfo>) derived from the T7 bacteriophage allows high expression of proteins. | Keratinase KerAVDZ50 (<partinfo>K3895003</partinfo>) is an extracellular serine thiol alkaline protease from the ''Actinomadura viridilutea'' strain, which can be isolated from the Algerian fishing port [1]. As a thermo-alkaline keratinases, it can function at a pH range of 7–12 and a temperature range of 35–80 °C. 6x His-tags were added to both sides of the sequence for purification and constructed into PET-28a(+). The constitutive T7 promoter (<partinfo>I719005</partinfo>) derived from the T7 bacteriophage allows high expression of proteins. | ||
| − | [[File:T--SZ SHD--Z50plasmid.jpg| | + | [[File:T--SZ SHD--Z50plasmid.jpg|400px|center]] |
Revision as of 07:44, 15 October 2021
kerAvDZ50_dna_pET-28a(+)
Construction
Keratinase KerAVDZ50 (BBa_K3895003) is an extracellular serine thiol alkaline protease from the Actinomadura viridilutea strain, which can be isolated from the Algerian fishing port [1]. As a thermo-alkaline keratinases, it can function at a pH range of 7–12 and a temperature range of 35–80 °C. 6x His-tags were added to both sides of the sequence for purification and constructed into PET-28a(+). The constitutive T7 promoter (BBa_I719005) derived from the T7 bacteriophage allows high expression of proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 606
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 411
Illegal AgeI site found at 1149 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 351