Difference between revisions of "Part:BBa E0051:Design"
| Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | amino acids | + | amino acids 1-60 from lacZ and amino acids 2-end of GFP (E0043) |
===References=== | ===References=== | ||
Revision as of 19:18, 2 March 2009
lacZa.GFP fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 878
Design Notes
The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc. The start codon from GFP was removed.
Source
amino acids 1-60 from lacZ and amino acids 2-end of GFP (E0043)