Difference between revisions of "Part:BBa K3982030:Design"

Revision as of 19:28, 14 October 2021

CODE M Construct C2

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 124
Illegal XbaI site found at 148
Illegal PstI site found at 160
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 124
Illegal PstI site found at 160
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 124
Illegal BglII site found at 598
Illegal BamHI site found at 142
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 124
Illegal XbaI site found at 148
Illegal PstI site found at 160
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 124
Illegal XbaI site found at 148
Illegal PstI site found at 160
1000
COMPATIBLE WITH RFC[1000]

Design Notes

sgRNA sequence BBa_K3982004 inserted using SDM

Source

Addgene #15030

References

1) Kim, D.Y., Lee, J.M., Moon, S.B. et al. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-01009-z

2) Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science (New York, N.Y.), 362(6416), 839–842. https://doi.org/10.1126/science.aav4294

@@ Line 13: / Line 13: @@
 ===References===
+) Kim, D.Y., Lee, J.M., Moon, S.B. et al. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-01009-z
+) Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science (New York, N.Y.), 362(6416), 839–842. https://doi.org/10.1126/science.aav4294