Difference between revisions of "Part:BBa K3982030:Design"
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===Design Notes===
 
===Design Notes===
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sgRNA sequence [https://parts.igem.org/Part:BBa_K3982004/ BBa_K3982004] inserted using SDM
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===Source===
 
===Source===
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[https://www.addgene.org/15030// Addgene #15030]
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Addgene 15030
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===References===
 
===References===

Revision as of 18:31, 14 October 2021


CODE M Construct C2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 124
    Illegal XbaI site found at 148
    Illegal PstI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 124
    Illegal PstI site found at 160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 124
    Illegal BglII site found at 598
    Illegal BamHI site found at 142
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 124
    Illegal XbaI site found at 148
    Illegal PstI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 124
    Illegal XbaI site found at 148
    Illegal PstI site found at 160
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sgRNA sequence BBa_K3982004 inserted using SDM

Source

Addgene #15030

References