Difference between revisions of "Part:BBa K3970027"

 
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==Construction==
 
==Construction==
 
[[File:0028-1.png|600px|thumb|center]]
 
[[File:0028-1.png|600px|thumb|center]]
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==Sequence==
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<p style="word-wrap:break-word;">CTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGATGGTTAGAGTTGCTATTAACGGTTTCGGTAGAATCGGTAGATTGGTCATGAGAATTGCTTTGTCTAGACCAAACGTCGAAGTTGTTGCTTTGAACGACCCATTCATCACCAACGACTACGCTGCTTACATGTTCAAGTACGACTCCACTCACGGTAGATACGCTGGTGAAGTTTCCCACGATGACAAGCACATCATTGTCGATGGTAAGAAGATTGCTACTTACCAAGAAAGAGACCCAGCTAACTTGCCATGGGGTTCTTCCAACGTTGACATCGCCATTGACTCCACTGGTGTTTTCAAGGAATTAGACACTGCTCAAAAGCACATTGACGCTGGTGCCAAGAAGGTTGTTATCACTGCTCCATCTTCCACCGCCCCAATGTTCGTCATGGGTGTTAACGAAGAAAAATACACTTCTGACTTGAAGATTGTTTCCAACGCTTCTTGTACCACCAACTGTTTGGCTCCATTGGCCAAGGTTATCAACGATGCTTTCGGTATTGAAGAAGGTTTGATGACCACTGTCCACTCTTTGACTGCTACTCAAAAGACTGTTGACGGTCCATCCCACAAGGACTGGAGAGGTGGTAGAACCGCTTCCGGTAACATCATCCCATCCTCCACCGGTGCTGCTAAGGCTGTCGGTAAGGTCTTGCCAGAATTGCAAGGTAAGTTGACCGGTATGGCTTTCAGAGTCCCAACCGTCGATGTCTCCGTTGTTGACTTGACTGTCAAGTTGAACAAGGAAACCACCTACGATGAAATCAAGAAGGTTGTTAAGGCTGCCGCTGAAGGTAAGTTGAAGGGTGTTTTGGGTTACACCGAAGACGCTGTTGTCTCCTCTGACTTCTTGGGTGACTCTCACTCTTCCATCTTCGATGCTTCCGCTGGTATCCAATTGTCTCCAAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTACCAGAGTTGTCGACTTGGTTGAACACGTTGCCAAGGCTTAAATGAAGACCCCTATCACCGAAGCCATTGCCGCTGCTGACACTCAAGGCCGTTTTTTGTCCAACACTGAATTGCAAGCTGCTGATGGTAGATTCAAGAGAGCTGTTGCTTCCATGGAAGCTGCCAGAGCTTTGACCAACAACGCCCAATCTTTGATTGATGGTGCTGCACAAGCCGTCTACCAAAAGTTCCCATACACTACCACCATGCAAGGTTCTCAATATGCTTCTACTCCAGAAGGTAAGGCTAAGTGTGCTAGAGATATCGGTTACTACTTGAGAATGGTTACATACTGTTTGGTCGCCGGTGGTACTGGTCCAATGGACGAATACTTAATTGCTGGTTTGGCTGAAATCAACTCCACTTTCGACTTGTCTCCATCTTGGTACGTTGAAGCTTTGAAGTACATCAAAGCCAACCACGGTTTATCAGGTCAAGCTGCTGTCGAAGCTAACGCTTACATTGACTACGCTATCAATGCTCTATCCTGAgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccgg</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 14:48, 14 October 2021


LexO-cpTDH3-CpcA-tADH1

Usage and Biology

This part is the effector of the upstream regulator, the product of the LexA will bind to the following LexO part and active the downstream cpcA gene expression. With the regulation of cpcA, phycocyanin a-subunit will not effect the growth of yeast.

Construction

0028-1.png

Sequence

CTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGATGGTTAGAGTTGCTATTAACGGTTTCGGTAGAATCGGTAGATTGGTCATGAGAATTGCTTTGTCTAGACCAAACGTCGAAGTTGTTGCTTTGAACGACCCATTCATCACCAACGACTACGCTGCTTACATGTTCAAGTACGACTCCACTCACGGTAGATACGCTGGTGAAGTTTCCCACGATGACAAGCACATCATTGTCGATGGTAAGAAGATTGCTACTTACCAAGAAAGAGACCCAGCTAACTTGCCATGGGGTTCTTCCAACGTTGACATCGCCATTGACTCCACTGGTGTTTTCAAGGAATTAGACACTGCTCAAAAGCACATTGACGCTGGTGCCAAGAAGGTTGTTATCACTGCTCCATCTTCCACCGCCCCAATGTTCGTCATGGGTGTTAACGAAGAAAAATACACTTCTGACTTGAAGATTGTTTCCAACGCTTCTTGTACCACCAACTGTTTGGCTCCATTGGCCAAGGTTATCAACGATGCTTTCGGTATTGAAGAAGGTTTGATGACCACTGTCCACTCTTTGACTGCTACTCAAAAGACTGTTGACGGTCCATCCCACAAGGACTGGAGAGGTGGTAGAACCGCTTCCGGTAACATCATCCCATCCTCCACCGGTGCTGCTAAGGCTGTCGGTAAGGTCTTGCCAGAATTGCAAGGTAAGTTGACCGGTATGGCTTTCAGAGTCCCAACCGTCGATGTCTCCGTTGTTGACTTGACTGTCAAGTTGAACAAGGAAACCACCTACGATGAAATCAAGAAGGTTGTTAAGGCTGCCGCTGAAGGTAAGTTGAAGGGTGTTTTGGGTTACACCGAAGACGCTGTTGTCTCCTCTGACTTCTTGGGTGACTCTCACTCTTCCATCTTCGATGCTTCCGCTGGTATCCAATTGTCTCCAAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTACCAGAGTTGTCGACTTGGTTGAACACGTTGCCAAGGCTTAAATGAAGACCCCTATCACCGAAGCCATTGCCGCTGCTGACACTCAAGGCCGTTTTTTGTCCAACACTGAATTGCAAGCTGCTGATGGTAGATTCAAGAGAGCTGTTGCTTCCATGGAAGCTGCCAGAGCTTTGACCAACAACGCCCAATCTTTGATTGATGGTGCTGCACAAGCCGTCTACCAAAAGTTCCCATACACTACCACCATGCAAGGTTCTCAATATGCTTCTACTCCAGAAGGTAAGGCTAAGTGTGCTAGAGATATCGGTTACTACTTGAGAATGGTTACATACTGTTTGGTCGCCGGTGGTACTGGTCCAATGGACGAATACTTAATTGCTGGTTTGGCTGAAATCAACTCCACTTTCGACTTGTCTCCATCTTGGTACGTTGAAGCTTTGAAGTACATCAAAGCCAACCACGGTTTATCAGGTCAAGCTGCTGTCGAAGCTAACGCTTACATTGACTACGCTATCAATGCTCTATCCTGAgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccgg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]