Difference between revisions of "Part:BBa K3970028"
 
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==Sequence==
 
==Sequence==
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CTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGATGGTTAGAGTTGCTATTAACGGTTTCGGTAGAATCGGTAGATTGGTCATGAGAATTGCTTTGTCTAGACCAAACGTCGAAGTTGTTGCTTTGAACGACCCATTCATCACCAACGACTACGCTGCTTACATGTTCAAGTACGACTCCACTCACGGTAGATACGCTGGTGAAGTTTCCCACGATGACAAGCACATCATTGTCGATGGTAAGAAGATTGCTACTTACCAAGAAAGAGACCCAGCTAACTTGCCATGGGGTTCTTCCAACGTTGACATCGCCATTGACTCCACTGGTGTTTTCAAGGAATTAGACACTGCTCAAAAGCACATTGACGCTGGTGCCAAGAAGGTTGTTATCACTGCTCCATCTTCCACCGCCCCAATGTTCGTCATGGGTGTTAACGAAGAAAAATACACTTCTGACTTGAAGATTGTTTCCAACGCTTCTTGTACCACCAACTGTTTGGCTCCATTGGCCAAGGTTATCAACGATGCTTTCGGTATTGAAGAAGGTTTGATGACCACTGTCCACTCTTTGACTGCTACTCAAAAGACTGTTGACGGTCCATCCCACAAGGACTGGAGAGGTGGTAGAACCGCTTCCGGTAACATCATCCCATCCTCCACCGGTGCTGCTAAGGCTGTCGGTAAGGTCTTGCCAGAATTGCAAGGTAAGTTGACCGGTATGGCTTTCAGAGTCCCAACCGTCGATGTCTCCGTTGTTGACTTGACTGTCAAGTTGAACAAGGAAACCACCTACGATGAAATCAAGAAGGTTGTTAAGGCTGCCGCTGAAGGTAAGTTGAAGGGTGTTTTGGGTTACACCGAAGACGCTGTTGTCTCCTCTGACTTCTTGGGTGACTCTCACTCTTCCATCTTCGATGCTTCCGCTGGTATCCAATTGTCTCCAAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTACCAGAGTTGTCGACTTGGTTGAACACGTTGCCAAGGCTTAAATGTTGTCATTAGAACAATTGGCTACCCAATTGGAATCTCCAAACTCTAGAGACAGATTGTTGGCCCTTGCTGCCTTAAGAGATGTTCCACCTCCAGAAGCTGTTCCATTGATCAAGAAGGTTTTGTGGGATGACAATTTGCAAATCCGTTCCATGGCTGTCTTTGCTTTGGGTGTTAAGCCTACCCCAGAATGTTTCGACATTTTAGTCCAATTGTTGGAAACTGAACCAGACTACGGTATCAGAGCTGATGCTGCTGGTGCCTTGGGTTATTTGCAAGACCAAAGAGCCTTCGCTCCATTAGTCAGAGCTTTCTACGAAGACACTGATTGGTTGGTTAGATTCTCTGCCGCTGTTTCCTTAGGTAACTTGAAAGATGTCCGTGCTGTCGAAGTCTTGAGATCTGCTTTGTCCTCTAACGAGGTTGTTATGTTGCAAGGTGCTATTGCTGCTTTGGGTGAAATTGGTGACCCATCCATTGTTGAAGAAATCTTGCCATTCGTCACTGCTGAAGACTGGTTGATTAGACAAAGATTAGCCGAAGCCCTAGGTAACTTGCCATCTCCAAAGACCCAATCCGCACTAGAATACTTGGCTAAGGATGAACACCCACACGTTGCTGCCGCTGCTGAAATCTCTTTGCAACGTTTGGCTCAAAGATGAtggtccgagtcatgtaattagttatgtcacgcttacGttcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgc
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<p style="word-wrap:break-word;">CTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGATGGTTAGAGTTGCTATTAACGGTTTCGGTAGAATCGGTAGATTGGTCATGAGAATTGCTTTGTCTAGACCAAACGTCGAAGTTGTTGCTTTGAACGACCCATTCATCACCAACGACTACGCTGCTTACATGTTCAAGTACGACTCCACTCACGGTAGATACGCTGGTGAAGTTTCCCACGATGACAAGCACATCATTGTCGATGGTAAGAAGATTGCTACTTACCAAGAAAGAGACCCAGCTAACTTGCCATGGGGTTCTTCCAACGTTGACATCGCCATTGACTCCACTGGTGTTTTCAAGGAATTAGACACTGCTCAAAAGCACATTGACGCTGGTGCCAAGAAGGTTGTTATCACTGCTCCATCTTCCACCGCCCCAATGTTCGTCATGGGTGTTAACGAAGAAAAATACACTTCTGACTTGAAGATTGTTTCCAACGCTTCTTGTACCACCAACTGTTTGGCTCCATTGGCCAAGGTTATCAACGATGCTTTCGGTATTGAAGAAGGTTTGATGACCACTGTCCACTCTTTGACTGCTACTCAAAAGACTGTTGACGGTCCATCCCACAAGGACTGGAGAGGTGGTAGAACCGCTTCCGGTAACATCATCCCATCCTCCACCGGTGCTGCTAAGGCTGTCGGTAAGGTCTTGCCAGAATTGCAAGGTAAGTTGACCGGTATGGCTTTCAGAGTCCCAACCGTCGATGTCTCCGTTGTTGACTTGACTGTCAAGTTGAACAAGGAAACCACCTACGATGAAATCAAGAAGGTTGTTAAGGCTGCCGCTGAAGGTAAGTTGAAGGGTGTTTTGGGTTACACCGAAGACGCTGTTGTCTCCTCTGACTTCTTGGGTGACTCTCACTCTTCCATCTTCGATGCTTCCGCTGGTATCCAATTGTCTCCAAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTACCAGAGTTGTCGACTTGGTTGAACACGTTGCCAAGGCTTAAATGTTGTCATTAGAACAATTGGCTACCCAATTGGAATCTCCAAACTCTAGAGACAGATTGTTGGCCCTTGCTGCCTTAAGAGATGTTCCACCTCCAGAAGCTGTTCCATTGATCAAGAAGGTTTTGTGGGATGACAATTTGCAAATCCGTTCCATGGCTGTCTTTGCTTTGGGTGTTAAGCCTACCCCAGAATGTTTCGACATTTTAGTCCAATTGTTGGAAACTGAACCAGACTACGGTATCAGAGCTGATGCTGCTGGTGCCTTGGGTTATTTGCAAGACCAAAGAGCCTTCGCTCCATTAGTCAGAGCTTTCTACGAAGACACTGATTGGTTGGTTAGATTCTCTGCCGCTGTTTCCTTAGGTAACTTGAAAGATGTCCGTGCTGTCGAAGTCTTGAGATCTGCTTTGTCCTCTAACGAGGTTGTTATGTTGCAAGGTGCTATTGCTGCTTTGGGTGAAATTGGTGACCCATCCATTGTTGAAGAAATCTTGCCATTCGTCACTGCTGAAGACTGGTTGATTAGACAAAGATTAGCCGAAGCCCTAGGTAACTTGCCATCTCCAAAGACCCAATCCGCACTAGAATACTTGGCTAAGGATGAACACCCACACGTTGCTGCCGCTGCTGAAATCTCTTTGCAACGTTTGGCTCAAAGATGAtggtccgagtcatgtaattagttatgtcacgcttacGttcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgc</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 14:46, 14 October 2021


LexO-cpTDH3-CpcE-tCYC1

Usage and Biology

This part is the effector of the upstream regulator, the product of the LexA will bind to the following LexO part and active the downstream cpcE gene expression. With the regulation of cpcE, the lyase a-subunit will not effect the growth of yeast.

Construction

0028-1.png

Sequence

CTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGATGGTTAGAGTTGCTATTAACGGTTTCGGTAGAATCGGTAGATTGGTCATGAGAATTGCTTTGTCTAGACCAAACGTCGAAGTTGTTGCTTTGAACGACCCATTCATCACCAACGACTACGCTGCTTACATGTTCAAGTACGACTCCACTCACGGTAGATACGCTGGTGAAGTTTCCCACGATGACAAGCACATCATTGTCGATGGTAAGAAGATTGCTACTTACCAAGAAAGAGACCCAGCTAACTTGCCATGGGGTTCTTCCAACGTTGACATCGCCATTGACTCCACTGGTGTTTTCAAGGAATTAGACACTGCTCAAAAGCACATTGACGCTGGTGCCAAGAAGGTTGTTATCACTGCTCCATCTTCCACCGCCCCAATGTTCGTCATGGGTGTTAACGAAGAAAAATACACTTCTGACTTGAAGATTGTTTCCAACGCTTCTTGTACCACCAACTGTTTGGCTCCATTGGCCAAGGTTATCAACGATGCTTTCGGTATTGAAGAAGGTTTGATGACCACTGTCCACTCTTTGACTGCTACTCAAAAGACTGTTGACGGTCCATCCCACAAGGACTGGAGAGGTGGTAGAACCGCTTCCGGTAACATCATCCCATCCTCCACCGGTGCTGCTAAGGCTGTCGGTAAGGTCTTGCCAGAATTGCAAGGTAAGTTGACCGGTATGGCTTTCAGAGTCCCAACCGTCGATGTCTCCGTTGTTGACTTGACTGTCAAGTTGAACAAGGAAACCACCTACGATGAAATCAAGAAGGTTGTTAAGGCTGCCGCTGAAGGTAAGTTGAAGGGTGTTTTGGGTTACACCGAAGACGCTGTTGTCTCCTCTGACTTCTTGGGTGACTCTCACTCTTCCATCTTCGATGCTTCCGCTGGTATCCAATTGTCTCCAAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTACCAGAGTTGTCGACTTGGTTGAACACGTTGCCAAGGCTTAAATGTTGTCATTAGAACAATTGGCTACCCAATTGGAATCTCCAAACTCTAGAGACAGATTGTTGGCCCTTGCTGCCTTAAGAGATGTTCCACCTCCAGAAGCTGTTCCATTGATCAAGAAGGTTTTGTGGGATGACAATTTGCAAATCCGTTCCATGGCTGTCTTTGCTTTGGGTGTTAAGCCTACCCCAGAATGTTTCGACATTTTAGTCCAATTGTTGGAAACTGAACCAGACTACGGTATCAGAGCTGATGCTGCTGGTGCCTTGGGTTATTTGCAAGACCAAAGAGCCTTCGCTCCATTAGTCAGAGCTTTCTACGAAGACACTGATTGGTTGGTTAGATTCTCTGCCGCTGTTTCCTTAGGTAACTTGAAAGATGTCCGTGCTGTCGAAGTCTTGAGATCTGCTTTGTCCTCTAACGAGGTTGTTATGTTGCAAGGTGCTATTGCTGCTTTGGGTGAAATTGGTGACCCATCCATTGTTGAAGAAATCTTGCCATTCGTCACTGCTGAAGACTGGTTGATTAGACAAAGATTAGCCGAAGCCCTAGGTAACTTGCCATCTCCAAAGACCCAATCCGCACTAGAATACTTGGCTAAGGATGAACACCCACACGTTGCTGCCGCTGCTGAAATCTCTTTGCAACGTTTGGCTCAAAGATGAtggtccgagtcatgtaattagttatgtcacgcttacGttcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgc


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]