Difference between revisions of "Part:BBa K3930008"
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<partinfo>BBa_K3930008 short</partinfo> | <partinfo>BBa_K3930008 short</partinfo> | ||
− | This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the Lavandula angustifolia genome, and has been codon optimized for expression into S.cerevisiae. | + | This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the <i>Lavandula angustifolia</i> genome, and has been codon optimized for expression into S.cerevisiae. |
+ | <html> | ||
+ | <h2>Introduction</h2> | ||
+ | <p>This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the <i>Lavandula angustifolia</i> genome. We take advantage of the publication of (Amiri et al. 2016). | ||
+ | <p>There is already BBa_K3052002 on the registry for linalool synthase. This part is codon optimized for translation in E. coli, but ours is codon optimized for translation in S. cerevisiae.</p> | ||
+ | |||
+ | |||
+ | <br> | ||
+ | <h2>References</h2> | ||
+ | <ol> | ||
+ | <i> | ||
+ | <li>Amiri P, Shahpiri A, Asadollahi MA, Momenbeik F, Partow S. 2016. Metabolic engineering of Saccharomyces cerevisiae for linalool production. Biotechnol Lett. 38(3):503–508. doi:10.1007/s10529-015-2000-4. | ||
+ | </li> | ||
+ | </i> | ||
+ | </ol> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 13:51, 14 October 2021
Gene coding for the Linalool synthase
This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the Lavandula angustifolia genome, and has been codon optimized for expression into S.cerevisiae.
Introduction
This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the Lavandula angustifolia genome. We take advantage of the publication of (Amiri et al. 2016).
There is already BBa_K3052002 on the registry for linalool synthase. This part is codon optimized for translation in E. coli, but ours is codon optimized for translation in S. cerevisiae.
References
- Amiri P, Shahpiri A, Asadollahi MA, Momenbeik F, Partow S. 2016. Metabolic engineering of Saccharomyces cerevisiae for linalool production. Biotechnol Lett. 38(3):503–508. doi:10.1007/s10529-015-2000-4.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 1000COMPATIBLE WITH RFC[1000]