Difference between revisions of "Part:BBa K3927002"

 
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<partinfo>BBa_K3927002 short</partinfo>
 
<partinfo>BBa_K3927002 short</partinfo>
  
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This part is an improvement of the existing part BBa_K1159105 where an NLS has been added to improve nuclear localization and endonuclease activity.
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This part encodes for a truncated CYCp core promoter with three C120 repeats replacing the native upstream activating sequence, and a lacO sequence downstream of the TATA box.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3927002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3927002 SequenceAndFeatures</partinfo>
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===Description===
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===Usage===
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===Design===
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===Characterization===
  
  

Revision as of 23:16, 13 October 2021


3C120-CYC-LacO

This part encodes for a truncated CYCp core promoter with three C120 repeats replacing the native upstream activating sequence, and a lacO sequence downstream of the TATA box.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 203
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 185
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 203
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 203
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

Usage

Design

Characterization