Difference between revisions of "Part:BBa K3853013"

Revision as of 23:08, 13 October 2021

MnP (E74P)

Manganese peroxidase (MnP) is the key enzyme in our degrading system. In order to improve its catalyzing ability, we tried rational design. And according to the computational redesign results, 6 mutants were chosen and tested, including their relative enzyme activity and the effect of temperature/pH/organic solvents on them. MnP(E74P) is one of the most promising mutant of MnP. We use BBa_K3853056 to construct the expression system and to express and to purify the protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3853013 short</partinfo>
-Manganese peroxidase (MnP) is the key enzyme in our degrading system. In order to improve its catalyzing ability, we tried rational design. And according to the computational redesign results, 6 mutants were chosen and tested, including their relative enzyme activity and the effect of temperature/pH/organic solvents on them. MnP(E74P) is the most promising mutant of MnP, possessing the lowest energy after mutation and would probably has the best stability, but finally showed a sharp decline in enzyme activity, even close to zero.
+Manganese peroxidase (MnP) is the key enzyme in our degrading system. In order to improve its catalyzing ability, we tried rational design. And  according to the computational redesign results, 6 mutants were chosen  and tested, including their relative enzyme activity and the effect of  temperature/pH/organic solvents on them. MnP(E74P) is one of the most promising mutant of MnP.  We use <partinfo>BBa_K3853056</partinfo>  to construct the expression system and to express and to purify the protein.
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