Difference between revisions of "Part:BBa K3941004"

Revision as of 19:51, 13 October 2021

pET29b+CelAB

BBa_K3941004 is a sequence that we used in our expression plasmids (pET29b). We produce CelAB via this specific sequence.

This composite part is made out of 4 different parts: - T7 Promoter (BBa_K3941000) - LacO (BBa_K1624002) - CelAB (BBa_K3941000) - T7 Terminator

Figure 1: T7 promoter, Lac Operon, Codon optimized CelAB, 6XHis and terminator

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 51
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 930
Illegal XhoI site found at 972
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 51
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 51
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3941004 short</partinfo>
-.
+BBa_K3941004 is a sequence that we used in our expression plasmids (pET29b). We produce CelAB via this specific sequence.
+This composite part is made out of 4 different parts:
+- T7 Promoter (BBa_K3941000)
+- LacO (BBa_K1624002)
+- CelAB (BBa_K3941000)
+- T7 Terminator
+https://static.igem.org/mediawiki/parts/7/77/T--Saint_Joseph--Diagram-CelAB-Whole.png
+<font size="-2"><b>Figure 1:</b> T7 promoter, Lac Operon, Codon optimized CelAB, 6XHis and terminator</font>
 <!-- Add more about the biology of this part here