Difference between revisions of "Part:BBa K3853012"
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<partinfo>BBa_K3853012 short</partinfo> | <partinfo>BBa_K3853012 short</partinfo> | ||
− | This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). This part of the amino acid sequence is the same as <partinfo>BBa_I746916</partinfo>. Using the <b>Thermo GeneArt</b> online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in <i>Pichia pastoris</i>. | + | This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). This part of the amino acid sequence is the same as <partinfo>BBa_I746916</partinfo>. Using the <b>Thermo GeneArt</b> online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in <i>Pichia pastoris</i>. We use <partinfo>BBa_3853055</partinfo> to construct the expression system and to express and to purify the protein. |
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Revision as of 17:41, 13 October 2021
sfGFP Codon adapted for Pichia pastoris
This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). This part of the amino acid sequence is the same as BBa_I746916. Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in Pichia pastoris. We use No part name specified with partinfo tag. to construct the expression system and to express and to purify the protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]