Difference between revisions of "Part:BBa K3930006"

 
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<partinfo>BBa_K3930006 short</partinfo>
 
<partinfo>BBa_K3930006 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3930006 SequenceAndFeatures</partinfo>
  
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This sequence aload the production of the activator Z3eV, which can activate the promoter Z3eV upon binding to estradiol.
 
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This sequence must be used when a plasmidic construction uses the promoter Z3eV from part (BBa_K3930007)
 
  
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<h2>Introduction</h2>
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<p>The Z3eV assembly  is composed by the promoter TEF1, the gene <i>Z3eV</i> coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al. 2020). </p>
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<p>This sequence must be used with the promoter Z3eV (BBa_K3930007), for a gene regulated expression into <i>S.cerevisiae</i>.
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K3930006 SequenceAndFeatures</partinfo>
 
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<!-- Uncomment this to enable Functional Parameter display  
 
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Revision as of 09:19, 13 October 2021


Inducer Z3EV of the estradiol promoter Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
    Illegal AgeI site found at 1527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1849
    Illegal BsaI.rc site found at 987


Introduction

The Z3eV assembly is composed by the promoter TEF1, the gene Z3eV coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al. 2020).

This sequence must be used with the promoter Z3eV (BBa_K3930007), for a gene regulated expression into S.cerevisiae.