Difference between revisions of "Part:BBa K3822003"
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<partinfo>BBa_K3822003 short</partinfo> | <partinfo>BBa_K3822003 short</partinfo> | ||
| − | The sequence of Hypermethylated region of TFPI2 | + | The sequence of Hypermethylated region of TFPI2.We use this part for detection of colorectal cancer (CRC). |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | In our project, the trigger sequence is linked to this part by DNA amplification and was added into the cell free system together with the plasmid contain toehold sequence, the report fluorescent protein will be expressed. The visible color and fluorescent signal can indicate the methylation degree of the TFPI2 gene in CRC patients. | ||
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Revision as of 05:15, 13 October 2021
Hypermethylated region of TFPI2
The sequence of Hypermethylated region of TFPI2.We use this part for detection of colorectal cancer (CRC).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]