Difference between revisions of "Part:BBa K3930015"

 
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<partinfo>BBa_K3930015 short</partinfo>
 
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<span class='h3bb'>Sequence and Features</span>
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The promoter TEF1 is a constitutive promoter that comes from the Saccharomyces cerevisiae genome.
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<h2>Introduction</h2>
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<p>The promoter TEF1 is a constitutive promoter that comes from the plasmid p405TEF1 from Nicolas Buchler & Fred Cross (unpublished). The TEF1 promoter coming from the yeast <i>Y. lipolitica</i> is already described and used in part (BBa_K2117000), but ours comes from the <i>S. cerevisiae</i> genome. </p>
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<h2>Results</h2>
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<h3>Production of &beta;-ionone (BBa_K3930003)</h3>
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<p> The part (BBa_K3930002) was linearized and transformed into the <i>S.cerevisiae</i> LycoYeast strain. The production of &beta;-ionone was measured by GC-MS. Figure 1 shows the mass spectrum of a CrtY expressing strain.</p>
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                <b>Figure 1: </b> <b> Production of &beta;-ionone upon galctose activation</b>
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                <p>&beta;-ionone is produced in vivo by our strain when it is induced by galactose. On the right are presented the mass spectra that correspond between the standard and the observed peak</p>
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<p><b>This promoter TEF1  work under those lab conditions.</b><p>
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Revision as of 21:43, 12 October 2021


Constitutive promoter TEF1 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168

Introduction

The promoter TEF1 is a constitutive promoter that comes from the plasmid p405TEF1 from Nicolas Buchler & Fred Cross (unpublished). The TEF1 promoter coming from the yeast Y. lipolitica is already described and used in part (BBa_K2117000), but ours comes from the S. cerevisiae genome.

Results

Production of β-ionone (BBa_K3930003)

The part (BBa_K3930002) was linearized and transformed into the S.cerevisiae LycoYeast strain. The production of β-ionone was measured by GC-MS. Figure 1 shows the mass spectrum of a CrtY expressing strain.



Figure 1: Production of β-ionone upon galctose activation

β-ionone is produced in vivo by our strain when it is induced by galactose. On the right are presented the mass spectra that correspond between the standard and the observed peak


This promoter TEF1 work under those lab conditions.