Difference between revisions of "Part:BBa K3982002"
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Tobacco Etch Virus (TEV) protease is used in Project CODE M by [https://2021.igem.org/Team:IISER_Berhampur/ Team IISER_Berhampur 2021] | Tobacco Etch Virus (TEV) protease is used in Project CODE M by [https://2021.igem.org/Team:IISER_Berhampur/ Team IISER_Berhampur 2021] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
TEV protease is a highly specific protease from Tobacco Etch Virus. It is used for controlled cleavage of fusion proteins and thus, their easy purification in in-vitro and in-vivo experiments. The cleavage site is between Gln and Gly/Ser residues. | TEV protease is a highly specific protease from Tobacco Etch Virus. It is used for controlled cleavage of fusion proteins and thus, their easy purification in in-vitro and in-vivo experiments. The cleavage site is between Gln and Gly/Ser residues. | ||
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===Reference=== | ===Reference=== | ||
Revision as of 17:47, 12 October 2021
Tobacco Etch Virus (TEV) protease
Tobacco Etch Virus (TEV) protease is used in Project CODE M by Team IISER_Berhampur 2021
Usage and Biology
TEV protease is a highly specific protease from Tobacco Etch Virus. It is used for controlled cleavage of fusion proteins and thus, their easy purification in in-vitro and in-vivo experiments. The cleavage site is between Gln and Gly/Ser residues.
Reference
L. B. Harrington et al., Science, 10.1126/science.aav4294 (2018).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]