Difference between revisions of "Part:BBa K3982001:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This is the sequence of Cas14a1 gene directly derived from Addgene Plasmid 112502.
+
It contains EcoR1 and Pst1 sites. To clone or excise this part, preferably use XBa1 and Spe1 Restriction Enzymes.
−
 
+
  
  
 
===Source===
 
===Source===
  
−
It is derived from Addgene plasmid 112502
+
[https://www.addgene.org/112502//Addgene plasmid 112502]
  
 
===References===
 
===References===

Revision as of 15:52, 12 October 2021


Cas14a1 expression gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1357
    Illegal BamHI site found at 951
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
    Illegal AgeI site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It contains EcoR1 and Pst1 sites. To clone or excise this part, preferably use XBa1 and Spe1 Restriction Enzymes.


Source

plasmid 112502

References