Difference between revisions of "Part:BBa K3982001"
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This sequence encodes the Cas14a1 protein. It is a highly compact RNA guided nuclease used by Team IISER_Berhampur 2021 in Project CODE M. | This sequence encodes the Cas14a1 protein. It is a highly compact RNA guided nuclease used by Team IISER_Berhampur 2021 in Project CODE M. | ||
Revision as of 15:48, 12 October 2021
Cas14a1 expression gene
This sequence encodes the Cas14a1 protein. It is a highly compact RNA guided nuclease used by Team IISER_Berhampur 2021 in Project CODE M.
Usage and Biology
Cas14a1 (also called Un1Cas12f1) is a miniature cas protein used in CRISPR/Cas technology identified from archaebacteria and bacteriophages. It is a RNA guided nuclease that cleaves ssDNA without any Protospacer Adjacent Motif (PAM) specificity i.e. PAM independent ssDNA cleavage. This enables high-fidelity Single-nucleotide polymorphism (SNP) genotyping. It is guided by a single guide RNA (sgRNA) which has two components - CRISPR RNA (crRNA) and Trans-activating crispr RNA (tracrRNA).
To know more about how this part is used in project CODE M, visit our wikipage. IISER_Berhampur 2021
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1357
Illegal BamHI site found at 951 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313
Illegal AgeI site found at 607 - 1000COMPATIBLE WITH RFC[1000]