Difference between revisions of "Part:BBa K3982001"
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<partinfo>BBa_K3982001 short</partinfo>
 
<partinfo>BBa_K3982001 short</partinfo>
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This sequence encodes the Cas14a1 protein. It is a highly compact RNA guided nuclease used by Team IISER_Berhampur 2021 in Project CODE M.
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This sequence encodes the Cas14a1 protein. It is a highly compact RNA guided nuclease used by [https://2021.igem.org/Team:IISER_Berhampur Team IISER_Berhampur 2021]in Project CODE M.
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===Usage and Biology===
 
===Usage and Biology===
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Cas14a1 (also called Un1Cas12f1) is a miniature cas protein used in CRISPR/Cas technology identified from archaebacteria and bacteriophages. It is a RNA guided nuclease that cleaves ssDNA without any Protospacer Adjacent Motif (PAM) specificity i.e. PAM independent ssDNA cleavage. This enables high-fidelity Single-nucleotide polymorphism (SNP) genotyping. It is guided by a single guide RNA (sgRNA) which has two components - CRISPR RNA (crRNA) and Trans-activating crispr RNA (tracrRNA).
 
Cas14a1 (also called Un1Cas12f1) is a miniature cas protein used in CRISPR/Cas technology identified from archaebacteria and bacteriophages. It is a RNA guided nuclease that cleaves ssDNA without any Protospacer Adjacent Motif (PAM) specificity i.e. PAM independent ssDNA cleavage. This enables high-fidelity Single-nucleotide polymorphism (SNP) genotyping. It is guided by a single guide RNA (sgRNA) which has two components - CRISPR RNA (crRNA) and Trans-activating crispr RNA (tracrRNA).
  
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[https://2021.igem.org/Team:IISER_Berhampur Click here]to know more about how this part is used in project CODE M by Team IISER_Berhampur 2021.
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To know more about how this part is used in project CODE M, visit our wikipage. [https://2021.igem.org/Team:IISER_Berhampur/Team IISER_Berhampur 2021]
  
 
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Revision as of 15:47, 12 October 2021


Cas14a1 expression gene This sequence encodes the Cas14a1 protein. It is a highly compact RNA guided nuclease used by Team IISER_Berhampur 2021 in Project CODE M.

Usage and Biology

Cas14a1 (also called Un1Cas12f1) is a miniature cas protein used in CRISPR/Cas technology identified from archaebacteria and bacteriophages. It is a RNA guided nuclease that cleaves ssDNA without any Protospacer Adjacent Motif (PAM) specificity i.e. PAM independent ssDNA cleavage. This enables high-fidelity Single-nucleotide polymorphism (SNP) genotyping. It is guided by a single guide RNA (sgRNA) which has two components - CRISPR RNA (crRNA) and Trans-activating crispr RNA (tracrRNA).

To know more about how this part is used in project CODE M, visit our wikipage. IISER_Berhampur 2021

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1357
    Illegal BamHI site found at 951
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
    Illegal AgeI site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]