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Revision as of 14:15, 12 October 2021


C.reinhardtii chloroplast rbcL 3UTR

This part contains the 3'UTR for the rbcL gene found in Chlamydomonas reinhardtii chloroplast genome. This is coded as a level-0 3UTR/TERM Phytobrick, which together with other level-0 Phytobrick can form a desired transcriptional unit for transformation.


Usage and Biology

This is a level-0 3UTR/TERM Phytobrick. It can be used along with other level-0 Phytobrick promoters and CDS to create a transcriptional unit.

Caution: In chloroplast rbcL 3'UTR don't serve as transcription terminators as in bacteria, but as elements for transcript maturation/stabilisation. atpB 3'UTR can be used as an alternative. (on advice from Prof. Saul Purton - UCL)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 490
    Illegal XbaI site found at 478
    Illegal XbaI site found at 520
    Illegal SpeI site found at 514
    Illegal PstI site found at 28
    Illegal PstI site found at 496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 490
    Illegal NheI site found at 42
    Illegal SpeI site found at 514
    Illegal PstI site found at 28
    Illegal PstI site found at 496
    Illegal NotI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 490
    Illegal BamHI site found at 508
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 490
    Illegal XbaI site found at 478
    Illegal XbaI site found at 520
    Illegal SpeI site found at 514
    Illegal PstI site found at 28
    Illegal PstI site found at 496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 490
    Illegal XbaI site found at 478
    Illegal XbaI site found at 520
    Illegal SpeI site found at 514
    Illegal PstI site found at 28
    Illegal PstI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

References

Wannathong T, Waterhouse JC, Young REB, Economou CK, Purton S. New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 2016;100:5467-5477. doi:10.1007/s00253-016-7354-6.

Michel Goldschmidt-Clermont, Miche`le Rahire and Jean-David Rochaix. Redundant cis-acting determinants of 3¢ processingand RNA stability in the chloroplast rbcL mRNA ofChlamydomonas. The Plant Journal (2008) 53, 566–577. doi: 10.1111/j.1365-313X.2007.03365.x