Difference between revisions of "Part:BBa K3745040"

Revision as of 12:31, 12 October 2021

RhlABC + tags + scaffold

This plasmid contains rhlA,B,C as well as tags for each enzyme and scaffold to connect these three enzymes together, and it is the most essential plasmid in our protein scaffold project. We place the ORF of specific tags in front of each enzyme's ORF. These tags could bind with the corresponding domain on the scaffold and form a protein complex that can carry out the three needed reactions to synthesize rhamnolipid.

We adopt the ratio of each domain on the scaffold from "", we plan to fine-tune the ratio of the domain in the future.

By connecting rhlA,B,C together, we successfully improved the reaction efficiency of the enzymes that assemble rhamnolipid by maximizing the collision probability of substrates and enzymes as well as minimizing the time interview between each reaction, hence increase rhamnolipid yield.

For the detailed function of each enzyme, see BBa_K3745030

Sequence and Features