Difference between revisions of "Part:BBa K3745040"

Revision as of 11:25, 12 October 2021

RhlABC + tags + scaffold

This plasmid contains rhlA,B,C as well as tags for each enzyme and scaffold to connect these three enzymes together, and it is the most essential plasmid in our protein scaffold project. We place the ORF of specific tags in front of each enzyme's ORF. These tags could bind with the corresponding domain on the scaffold and form a protein complex that can carry out the three needed reactions to synthesize rhamnolipid.

Referring to "", we adjust the ratio of each domain on the scaffold protein to fine-tune the number of each enzyme and maximize the production efficiency of the reaction.

By connecting rhlA,B,C together, we successfully improved the reaction efficiency of the enzymes that assemble rhamnolipid by maximizing the collision probability of substrates and enzymes as well as minimizing the time interview between each reaction, hence increase rhamnolipid yield.

For the detailed function of each enzyme, see BBa_K3745030

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2281
Illegal BamHI site found at 6640
Illegal XhoI site found at 6649
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2149
Illegal NgoMIV site found at 2554
Illegal NgoMIV site found at 4123
Illegal NgoMIV site found at 5446
Illegal NgoMIV site found at 5761
Illegal NgoMIV site found at 6076
Illegal NgoMIV site found at 6391
Illegal AgeI site found at 3166
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 5676
Illegal BsaI site found at 5991
Illegal BsaI site found at 6306
Illegal BsaI site found at 6621
Illegal SapI.rc site found at 4262
Illegal SapI.rc site found at 5305

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3745040 short</partinfo>
-This plasmid contains rhlA,B,C as well as a tag and scaffold to connect these three enzymes together, and it is the most essential plasmid in our protein scaffold project. By connecting rhlA,B,C together, we successfully improved the reaction efficiency of the enzymes that assemble rhamnolipid by maximizing the collision probability of substrates and enzymes as well as minimizing the time interview between each reaction, hence increase rhamnolipid yield.
+This plasmid contains rhlA,B,C as well as tags for each enzyme and scaffold to connect these three enzymes together, and it is the most essential plasmid in our protein scaffold project. We place the ORF of specific tags in front of each enzyme's ORF. These tags could bind with the corresponding domain on the scaffold and form a protein complex that can carry out the three needed reactions to synthesize rhamnolipid.
+Referring to "", we adjust the ratio of each domain on the scaffold protein to fine-tune the number of each enzyme and maximize the production efficiency of the reaction.
+By connecting rhlA,B,C together, we successfully improved the reaction efficiency of the enzymes that assemble rhamnolipid by maximizing the collision probability of substrates and enzymes as well as minimizing the time interview between each reaction, hence increase rhamnolipid yield.
 For the detailed function of each enzyme, see BBa_K3745030