Difference between revisions of "Part:BBa K313010:Experience"

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<br><center><font size="1">Figure 4. MS2 binding mechanism</font></br>
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<br><center><font size="1">Figure 4. MS2 binding mechanism</font>
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===split EGFP===
 
===split EGFP===

Revision as of 10:28, 12 October 2021


We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

Applications of BBa_K313010

We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.

2D stucture

UNIQ73c42d4e1bc0306b-partinfo-00000000-QINU UNIQ73c42d4e1bc0306b-partinfo-00000001-QINU
We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:

MS2 aptamer on the circRNA scaffold.png
Figure 1.2D stucture predicted by RNAfold

SYSU-CHINA circRNA-1.png
Figure 2.2D stucture predicted by StructureEditor

3D stucture

UNIQ73c42d4e1bc0306b-partinfo-00000002-QINU UNIQ73c42d4e1bc0306b-partinfo-00000003-QINU
We use this website:http://biophy.hust.edu.cn/new/3dRNA/create, to predict the 3D structure of our circRNA, which contains MS2 aptamer.The result lists below:


SYSU-CHINA circRNA-2.png
</br>
Figure 3. 3D stucture of MS2 aptamer predicted by Xiaolab
</br>

MS2 binding mechanism

UNIQ73c42d4e1bc0306b-partinfo-00000004-QINU UNIQ73c42d4e1bc0306b-partinfo-00000005-QINU


MS2 binding mechanism.png

Figure 4. MS2 binding mechanism

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split EGFP

UNIQ73c42d4e1bc0306b-partinfo-00000006-QINU UNIQ73c42d4e1bc0306b-partinfo-00000007-QINU
We are SYSU-CHINA,and this year we use this part as MS2 coat protein's aptamer to do a split EGFP experiment,for the proof of concept.Here we found a research article, which use liner RNA with MS2 and PP7 aptamer, to do the split EGFP experiment.Below are their experimental data.


The principle of spite EGFP.png

<center>Figure 3.the principle and experimental data of split EGFP